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1、JGVPapersinPress.PublishedFebruary9,2015asdoi:10.1099/vir.0.000084JournalofGeneralVirologyGenotypingcoronavirusesassociatedwithfelineinfectiousperitonitis--ManuscriptDraft--ManuscriptNumber:JGV-D-14-00253R1FullTitle:GenotypingcoronavirusesassociatedwithfelineinfectiousperitonitisShortTitle:FCoVinc

2、atswithfelineinfectiousperitonitisArticleType:StandardSection/Category:Animal-Positive-strandRNAVirusesCorrespondingAuthor:StuartG.Siddell,BScPhDUniversityofBristolBristol,UNITEDKINGDOMFirstAuthor:CatherineS.LewisOrderofAuthors:CatherineS.LewisEmilyPorterDavidMatthewsAnjaKiparSeverineTaskerChristo

3、pherR.HelpsStuartG.Siddell,BScPhDAbstract:Felinecoronavirus(FCoV)infectionsareendemicamongstcatsworldwide.Themajorityofinfectionsareasymptomatic,orresultonlyinmildentericdisease.However,approximately5%ofcasesdevelopfelineinfectiousperitonitis(FIP),asystemicdiseasethatisafrequentcauseofdeathinyoung

4、cats.Inthisstudy,wereportthecompletecodinggenomesequencesofsixFCoVs;threefromfecalsamplesfromhealthycatsandthreefromtissuelesionsamplesfromcatswithconfirmedFIP.Thesixsampleswereobtainedoveraperiodofeightweeksatasingle-sitecatrescueandrehomingcenterintheUK.Wefoundaminoaciddifferencesarelocatedat44p

5、ositionsacrossanalignmentofthesixvirustranslatomesand,at21ofthesepositions,thedifferencesfullyorpartiallydiscriminatebetweenthegenomesderivedfromthefecalsamplesandthegenomesderivedfromtissuelesionsamples.Inthisstudy,twoaminoaciddifferencesfullydiscriminatethetwoclassesofgenomes;thesearebothlocated

6、intheS2domainofthevirussurfaceglycoproteingene.Wealsoidentifieddeletionsinthe3cproteinORFofgenomesfromtwooftheFIPsamples.OurresultssupportpreviousstudiesthatimplicateSproteinmutationsinthepathogenesisofFIP.PoweredbyEditorialManager?andProduXionManager?fromAriesSystemsCorporationManuscriptIncluding

7、References(Worddocument)ClickheretodownloadManuscriptIncludingReferences(Worddocument):Lewisetal24112014RevisionTexTabFigleg.docxGenotypingcoronavirusesassociatedwithfelineinfectiousperitonitis12132CatherineSLewi