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1、JVIAccepts,publishedonlineaheadofprinton29May2013J.Virol.doi:10.1128/JVI.01055-13Copyright?2013,AmericanSocietyforMicrobiology.AllRightsReserved.1JVI01055-13Revised2GeneticcharacterizationofBetacoronaviruslineageCvirusesinbats3revealedmarkedsequencediver

2、genceinthespikeproteinofPipistrellusbat4coronavirusHKU5inJapanesepipistrelle:implicationsontheoriginofthe5novelMiddleEastRespiratorySyndromeCoronavirus6a,b,c,dddd7SusannaK.P.Lau,KennethS.M.Li,AlanK.L.Tsang,CarolS.F.Lam,Shakeelddda,b,c,d,#8Ahmed,HonglinCh

3、en,Kwok-HungChan,PatrickC.Y.Woo,andKwok-Yunga,b,c,d,#9Yuen10a11StateKeyLaboratoryofEmergingInfectiousDiseases,ResearchCentreofInfectionandbcd12Immunology,CarolYuCentreforInfection,DepartmentofMicrobiology,The13UniversityofHongKong,HongKong,China1415Runni

4、ngtitle:lineageCbetacoronavirusesinbats1617Keywords:bats,human,beta,coronavirus,HKU5,MERS,EMC,spike,lesserbamboo,18Pipistrellus19#20Correspondingauthor.Mailingaddress:StateKeyLaboratoryofEmergingInfectious21Diseases,DepartmentofMicrobiology,TheUniversity

5、ofHongKong,Room423,22UniversityPathologyBuilding,QueenMaryHospital,HongKong,China.Phone:(852)12322554892.Fax:(852)28551241.E-mail:kyyuen@hkucc.hku.hk(KYYuen),24pcywoo@hkucc.hku.hk(PCYWoo)25Abstract:250words26Text:4672words27228ABSTRACT29WhilethenovelMidd

6、leEastRespiratorySyndromeCoronavirus(MERS-CoV)isclosely30relatedtoTylonycterisbatCoVHKU4(Ty-BatCoVHKU4)andPipistrellusbatCoV31HKU5(Pi-BatCoVHKU5)inbatsfromHongKong,andotherpotentiallineageC32betacoronavirusesinbatsfromAfrica,EuropeandAmerica,itsanimalori

7、ginremains33obscure.Tobetterunderstandtheroleofbatsinitsorigin,weexaminedthemolecular34epidemiologyandevolutionoflineageCbetacoronavirusesamongbats.Ty-BatCoV35HKU4andPi-BatCoVHKU5weredetectedin29%and25%ofalimentarysamplesfrom36lesserbamboobat(Tylonycteri

8、spachypus)andJapanesepipistrelle(Pipistrellusabramus)37respectively.SequencingoftheirRdRp,SandNgenesrevealedthatMERS-CoVismore38closelyrelatedtoPi-BatCoVHKU5inRdRp(92.1-92.3%aaidentities)buttoTy-39BatCoVHKU4inS(66.8-67.4%a