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1、VECTOR-BORNEANDZOONOTICDISEASESVolume13,Number7,2013aMaryAnnLiebert,Inc.DOI:10.1089/vbz.2012.1101CoronavirusesinSouthAfricanBats11,211MarikeGeldenhuys,JacquelineWeyer,LouisH.Nel,andWandaMarkotterAbstractRecentstudiesinseveralAfricancountrieshaveprovidedthe?rste

2、videnceforthepresenceofcoronavirusesinAfricanbats.Herewedescribe,forthe?rsttime,thedetectionofRNAof3uniquecoronavirusspeciesinthetissuesofSouthAfricanbats.KeyWords:Coronavirus—Alphacoronavirus—SouthAfrica—Bat.IntroductiondoubleheminestedRT-PCRassayafterdesignin

3、galpha-andbetacoronavirus–speci?cprimers(originallybasedonprim-hesevereacuterespiratorysyndrome(SARS)epi-TersfromWooetal.2005)targetingtheRNA-dependentRNAdemicinChinain2002,resultedin8422laboratorycon-polymerase(RdRp)gene(availableonrequest).PCRampli-?rmedhuman

4、caseswith916mortalities(Chan-Yeungandconswerepuri?edwiththeWizardSVgelandPCRClean-UpXu2003).Thecausativeagentwasanewlyemergentcor-System(Promega,USA)accordingtothemanufacturer’sin-onavirus(CoV)thatmayhaveoriginatedfromasimilarvirus,structionsandsequencedwiththe

5、BigDyeterminatorV3.1KitSARS-relatedCoV,discoveredintheChinesehorseshoebats(AppliedBiosystems,USA),editedinCLCBiomainwork-(Rhinolophusspp.)(Lauetal.2005).Thisstimulatedfurtherbenchv6(CLCbio,Denmark)andalignedusingClustalXininvestigationsintothepresenceanddiversi

6、tyofbatcor-BioEdit(Hall1999).Aneighbor-joiningphylogenetictreeonaviruses(BtCoV)globally,andthesestudieshavecon-wasconstructedwith1000bootstrapreplicatesinMEGA5comitantlyledtothedescriptionofvariousnovelBtCoVs(Tamuraetal.2011).(Poonetal.2005,Drexleretal.2010,Reu

7、skenetal.2010).StudiesofAfricanbatspecieshaverevealedthepresenceofbatcoronaviruses;uniquealphacoronavirusesrelatedtotheResultsandDiscussionhumancoronavirus229EaswellasBetacoronavirusmembersAtotalof113gastrointestinalspecimenswereanalyzedVector-BorneandZoonoticD

8、iseases2013.13:516-519.oftheSARS-Rh-BtCoV–relatedspeciesclusterhavebeenfromwhich3specimenstestedpositivelyforCoVRNA;identi?ed(Tongetal.2009,Pfefferleetal.2009,Quanetal.Neoro