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1、ArchVirol(2005)150:24832500DOI10.1007/s00705-005-0598-6ReplicationoffelinecoronavirusesinperipheralbloodmonocytesH.L.Dewerchin?,E.Cornelissen?,andH.J.NauwynckLaboratoryofVirology,FacultyofVeterinaryMedicine,GhentUniversity,Merelbeke,BelgiumReceivedApril18,2005;accep

2、tedMay23,2005PublishedonlineAugust1,2005cSpringer-Verlag2005Summary.Felineinfectiousperitonitisvirus(FIPV)(Coronaviridae)causesthemostlethalviralinfectionincats:FIP.Therelatedfelineentericcoronavirus(FECV)causesmildenteritis.Whythesefelinecoronavirusesmanifestsodif

3、-ferentlyinvivoisnotknown.Inthisstudy,infectionkinetics(titresandantigenexpression)ofFIPV79-1146,andFECV79-1683,weredeterminedinperipheralbloodmonocytesfrom3donorcatsandcomparedtothoseinCrandellfelinekidney(CrFK)cells.Theinfectionkineticsinmonocyteswerehostdependent

4、.Monocytesfrom1catwereresistanttobothFIPV-andFECV-infection.Mono-cytesfromtheother2catscouldinitiallybeinfectedbybothFIPVandFECVbutFIPVinfectionwassustainedinmonocytesofonlyonecat.FECV-infectionwasneversustainedandviralproductionwasupto100timeslowerthaninFIPV-infect

5、edmonocytes.InCrFKcells,FIPVandFECVinfectionkineticsdidnotdiffer.Inmonocytesofalargercatpopulation(n=19)the3infectionpatternswerealsofound.Consideringall22investigatedcats,3/22werenotsusceptibleforFIPVandFECV.TherestcouldbeinfectedwithFECVandFIPVbut10/22catshadmonoc

6、ytesthatonlysustainedFIPVinfectionand9/22sustainedneitherFIPVnorFECVinfection.IntroductionTwocoronavirusesaredescribedincats:felineinfectiousperitonitisvirus(FIPV)andfelineentericcoronavirus(FECV).Thesefelinecoronavirusesarespreadworld-wideandinfectcatsandothermembe

7、rsofthefamilyFelidae.AninfectionwithFECVisusuallysubclinical,exceptinyoungkittenswhereitmaycausemildtoseverediarrhoea[23].Incontrast,FIPVinfectioncausesachronicand?Bothauthorscontributedequally.2484H.L.Dewerchinetal.veryoftenfatalpleuritis/peritonitis.Itisthemostimp

8、ortantcauseofdeathofinfectiousoriginincats.TwoformsofFIPexist:theeffusiveorwetformwiththetypicaleffusionsinbodycavitiesandthelesscommonnon