《The application of SIMS to nutrient tracer studiesSIMSin plant physiology 在營養(yǎng)示蹤研究中的應(yīng)用 植物生理學(xué)》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。

1、BiolCell(1992)74,127-134127?Elsevier,ParisOriginalarticleTheapplicationofSIMStonutrienttracerstudiesinplantphysiologyDennisLazof,RichardWLinton,RichardJVolk,ThomasWRuftyUSDA-ARS,CRL,POB1168,Oxford,NC27565,USA(Received25November1991;accepted19December1991)Summary-

2、Currentcontroversiesinrootphysiologyrelatingtotheuptakeandtranslocationofmineralnutrientsarepresented.TheopportunitiesforaSIMScontributiontoresolvethesecontroversiesarediscussedforeachofthestableisotopetracersrelevanttoplantnutrition.Itisconcludedthatforallmajorn

3、utrientsexceptphosphorustherearepromisingstableisotopetracers.Atthesametime,therearechallengestoovercomeineachcase,withrespecttobackgroundlevels,peakinterferencesandsometimessensi-tivity.Techniquesoftissuepreparationandhandlingarediscussedinsomedetail,givingatten

4、tiontothespecialrequirementsofplanttissuepreparationandanalysisfordiffusableions.Itissuggestedthatadaptingapreparationandtransfersystem,designedforproductionofbulkfrozen-hydratedcryofracturedspecimens,toasecondaryionmassspectrometerwouldpermiteasy,rapidpreparatio

5、n.Additionally,thispreparationminimizesseveralserioustechnicalproblemsinherenttootherpreparativemethods.Difficul-tiesinquantitationaretreatedbriefly,outliningsomedifficultiesspecifictoplanttissueanalysis.Prospectsforfutureapplicationsinplantmineralnutritionareeva

6、luated,takingintoaccountcurrentinstrumentaldevelopments.cryotechniques/iontransport/plantphysiology/secondaryionmassspectrometry/stableisotopeMassspectrometryinplantnutritionexaminetransportof~SNinshort-termexperiments.Wehave,forexample,beenabletomeasureNO3-influ

7、xMassspectrometryhasnotbeenusedextensivelyinplantintosmallregionsofplantroots(ca1mgDW)fromdi-nutritionresearch.Whenuseditwasprimarilyrelatedtoluteexternalnutrientsolution(100~M[NO3]ext)overtheassimilationofcarbonandnitrogen.Forexample,Eh-time-periodsasbriefas1min

8、(LazofandRufty,unpub-leringerandOsmond's(1989)reviewofstableisotopelishedresults).Whilesensitivitywillalwaysbeordersof(SI)methodsinplantphysiologicalecologyonl