《Enhanced Sensitivity of the MRLMpJ Mouse to theNeuroplastic and Behavioral Effects of Chronic增強(qiáng)MRL_MPJ小鼠的靈敏度 慢性神經(jīng)行為和行為的影響 抗抑郁治療》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。

1、Neuropsychopharmacology(2009)34,1764–1773&2009NaturePublishingGroupAllrightsreserved0893-133X/09$32.00www.neuropsychopharmacology.orgEnhancedSensitivityoftheMRL/MpJMousetotheNeuroplasticandBehavioralEffectsofChronicAntidepressantTreatmentsDarrickTBalu1,GeorgiaEHodes2,BrianTAnderson2andIrwi

2、nLucki*,1,212DepartmentofPharmacology,UniversityofPennsylvania,Philadelphia,PA,USA;DepartmentofPsychiatry,UniversityofPennsylvania,Philadelphia,PA,USAChronicadministrationofantidepressantdrugsproducechangesinneuroplasticityandbehaviorinrodents,effectsthatmaybeassociatedwiththeslowemergence

3、ofclinicaltherapeuticeffects.Owingtotheuncertaintyovertheeffectsofchronicantidepressanttreatmentsinmice,theseexperimentscomparedtheregulationofneurogenesis,neurotrophinlevels,andbehaviorproducedbychronicantidepressanttreatmentsbetweentwoinbredmousestrains,MRL/MpJandC57BL/6J.TheMRL/MpJstrai

4、nisassociatedwithenhancedwoundhealingandtissueregeneration,whereasC57BL/6Jmiceareusedcommonlyforbehavioralstudies.Proliferationandsurvivalofhippocampalprogenitorcellsweremeasuredusingflowcytometry,anewplatformthatrapidlyquantifiestheincorporationof5-bromo-2-deoxyuridine(BrdU).Hippocampalce

5、llproliferationwasincreasedsignificantlyafterchronicadministrationoffluoxetine(FLX:5,10mg/kg,intraperitoneal(i.p.),b.i.d.)ordesipramine(DMI:5,10mg/kg,i.p.,b.i.d.)for21daysinMRL/MpJmice,butnotinC57BL/6Jmice.Hippocampalprogenitorcellsbornpriortochronicantidepressanttreatmentswerenotaffectedi

6、neithermousestrain.Proteinlevelsofbrain-derivedneurotrophicfactor(BDNF)inMRL/MpJmicewereelevatedsignificantlyinthefrontalcortex,hippocampus,andamygdalaafterchronicFLXtreatment,butincreasedonlyinthefrontalcortexbychronicDMI.Incontrast,BDNFlevelsinC57BL/6Jmiceweredecreasedinthehippocampusand

7、increasedintheamygdalaafterchronicFLX,andweredecreasedinthebrainstemafterchronicDMI.Novelty-inducedhypophagia(NIH)wasusedtoexamineabehavioraleffectproducedbychronicantidepressanttreatment.MRL/MpJmice,chronicallyadministeredFLXorDMI,hadsignificantlyshorterlaten