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1、AdvancePublicationTheJournalofVeterinaryMedicalScienceAcceptedDate:24October2019J-STAGEAdvancePublishedDate:5November2019?2019TheJapaneseSocietyofVeterinaryScienceAuthormanuscriptshavebeenpeerreviewedandacceptedforpublicationbuthavenotyetbeenedited.VirologyFullpa

2、perEvaluationofserologicalassaysavailableinabiosafetylevel2laboratoryandtheirapplicationforsurveyofMiddleEastRespiratorySyndromeCoronavirusamonglivestockinEthiopiaTakurouTeramichi1)＃ShuetsuFukushi2),YumaHachiya1),SimenewKeskesMelaku3),KeisukeOguma1),HiroshiSentsu

3、i1)*1)LaboratoryofVeterinaryEpizootiology,DepartmentofVeterinaryMedicine,NihonUniversity,Kameino1866,Fujisawa,Kanagawa252–0880,Japan2)DepartmentofVirologyI,NationalInstituteofInfectiousDiseases,1-23-1Toyama,Shinjyku,Tokyo162-8640,Japan3)EthiopiaAddisAbabaSciencea

4、ndTechnologyUniversity,AkakyKalitisub-city,Kilintoarea,AddisAbaba,Ethiopia*Correspondenceto:HiroshiSentsui,LaboratoryofVeterinaryEpizootiology,DepartmentofVeterinaryMedicine,NihonUniversity,Kameino1866,Fujisawa,Kanagawa252–0880,Japan.Tel:+81466843384;Fax:+8146684

5、3444;Email:sentsui.hiroshi@nihon-u.ac.jp＃PresentAddress:Teramichi,T.,IsahayaMeatInspectionCenter,79-20,Sakaimachi79-20,Isahaya,Nagasaki854-0022,Japan.RunningTitle:SEROLOGICALSURVEYOFMERS-CORONAVIRUS1ABSTRACTAserologicalsurveyofMiddleEastrespiratorysyndromecoronav

6、irus(MERS-CoV)wasconductedamongdromedarycamelsandherbivorousanimalssharingthesamepasturageinEthiopia.ThepseudotypedvesicularstomatitisviruscoatedwiththespikeproteinofMERS-CoVwasusedinvirusneutralization(VN)testsperformedinabiosafetylevel(BSL)-2laboratory.Theresul

7、tsweresimilartothoseobtainedfromtheVNtestusingliveMERS-CoVandweremoresensitivethantheELISAperformedusingsyntheticMERSS1fragmentastheantigenaswellasthecompetitiveELISAperformedusingamonoclonalantibodyagainstMERS-CoV.Accordingtothecomprehensiveresultsofthefourtypes

8、ofserodiagnosismethods,positiveantibodiesweredetectedonlyindromedarycamelsandtheremainingherbivorousanimalswerenotinfectedwiththevirus.Moreover,usingthepresent