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1、ResearchinVeterinaryScience1990.48.165-169SitesofreplicationofaporcinerespiratorycoronavirusrelatedtotransmissiblegastroenteritisvirusE.cox,J.HOOYBERGHS,M.B.PENSAERT,LaboratoryofVeterinaryVirologyandImmunology,Casinoplein24,B-9000Ghent,Belgium.Aporcinerespiratorycoro

2、navirus(PRCV)wasTheappearanceofaporcinecoronavirusrelatedtoinoculatedbyaerosolintoninehysterectomy-derivedTGEYwasobservedin1984intheswinepopulationinandcolostrum-deprivedpigsattheageofoneweek.Belgium(Pensaertetal1986).Thisvirushas,sinceTheywerekilledatdifferenttimesa

3、fterinoculationthen,becomewidespreadinseveralEuropeanandtissuesweresampledforvirusisolationandcountries(BrownandCartwright1986,Jestinetalimmunofluorescence.Resultsindicatethatvirus1987,vanNieuwstadtandPol1989).Itreplicatesinreplicatedtohightitresintherespiratorytract

4、.therespiratorytractandistransmittedaerogenically.ReplicationmainlyoccurredinalveolarcellsbutalsoAlthoughrespiratorydistresshasbeenreporteduponinepithelialcellsofnasalmucosa,trachea,bronchi,infectionwiththisporcinerespiratorycoronavirusbronchioli,inalveolarmacrophage

5、sandintonsils.(PRCY),itusuallycausesasubclinicalinfection.Afterprimaryreplicationintherespiratorytract,Infectionwiththisvirusdoesnotcausediarrhoeaviraemiaoccurred.Virusalsoreachedthegastro?(Pensaertetal1986,Jestinetal1987,Duretetalintestinaltractafterswallowing.Subse

6、quently,PRCV1988).wasobservedtoreplicateintheileum.TheinflftionRecently,theantigenicrelationshipbetweenthespreadwithinafewdaysfromtheileumtotheduo?BelgianisolateofPRCY,designatedTLM83,andthedenum.ReplicationinthesmallintestineremainedclassicalTGEYhasbeenstudied(Calle

7、bautetaI1988).limitedtoafewcellslocatedinorunderneaththeepi?Bothvirusesshowacompletecrossneutralisationtheliallayerofvilliand,or,crypts.Thecelltypecouldactivityandpossesscommonantigenicdeterminantsnotbeidentified.Viruswasisolatedfrommesentericoneachoftheirstructuralp

8、roteins.Nevertheless,lymphnodesinallpigs,butimmunofluorescencewasdistinctantigenicdifferenceshavebeendemonstratednotobserved.Result