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1、879MonoclonalAntibodytotheReceptorforMurineCoronavirusMHV-A59InhibitsViralReplicationInVivoAbigailL.Smith,ChristineB.Cardellichio,SectionofComparativeMedicineandDepartmentofEpidemiologyandDeborahF.Winograd,MarkS.deSouza,PublicHealth,YaleUniversity,NewHaven,Connecticut;Departme

2、ntpfStephenW.Barthold,andKathrynV.HolmesPathology,UniformedServicesUniversityoftheHealthSciences,Bethesda,MarylandBecausemanystrainsofmousehepatitisvirus(MHV)infectlaboratorymice,noeffectivevac?cinehasyetbeendeveloped.AnalternativeapproachtocontrolMHVdiseaseistheuseofahostcell

3、receptor-targetedligand.Toaddressthepotentialusefulnessofthisapproach,amono?clonalantibodydirectedagainstthehostcellreceptorforthecoronavirusMHV-A59wasad?ministeredtoinfantmicethatwerethenchallengedoronasallywith1()4intracerebralinfantmousemedianlethaldosesofMH'-A59.Antibodyt

4、reatmentofvirus-challengedmiceresultedinlowerproportionsofmicewithMHV-A59intargetorgansandmarkedlyreducedviraltitersintheseorganscomparedwithmock-treatedinfectedmice.Someantibody-treatedinfectedmicesurvivedfor7daysafterviralchallenge,whereasnomock-treated,infectedmicesurvivedb

5、e?yondday3afterviralinoculation.Theseresultssupportareceptor-targetedapproachtointer?ventionincoronavirusdisease.DownloadedfromViralreceptorsplayimportantrolesindeterminingthespe?[8,9].Comparablemembranepreparationsfromgeneticallyciesspecificity,tissuetropism,andpathogenesisof

6、animalresistantSJLmicedonotbindMHV-A59[8,9].Thesestudiesjid.oxfordjournals.orgviralinfectionsinvivo[1].ReceptorsforvirusesinmanysuggestthatgeneticallybasedsusceptibilitytoMHV-A59maydifferentgroupshavebeenrecentlyidentified,leadingtoeffortsbedetermined,atleastinpart,byexpressio

7、nofthevirus?toinhibitviralinfectioninvitroandinvivobyblockingvirus?bindingmoietyontheplasmamembranesofnormaltargetreceptorinteractions[2-5].Areceptor-targetedapproachap?tissuesforMHV.atUniversityofExeteronAugust16,2015pearsmostlikelytebeeffectiveinviraldiseasesinwhichApanelofm

8、onoc1onalantibodies(MAbs)reactivewiththethevirusentersthebody