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1、ArchivesofVirologyArchivesofVirology58,243--247(1978)?bySpringer-Verlag1978ANewCoronavirus-LikePartieleAssoeiatedWithDiarrheainSwine1BriefReportByM.B.PENSAERTandP.DEBOUCKLaboratoryofVirology,FacultyofVeterinaryMedicineUniversityofGent,Gent,BelgiumWith2FiguresAcceptedJune26,1978SummaryCoronav

2、irus-likeparticlesweredetectedbyelectronmicroscopyintheintestinalcontentsofpigsduringadiarrhealoutbreakon4swinebreedingfarms.Diarrheawasreproducedinexperimentalpigswithoneoftheisolates,designatedCV777,whichwasfoundtobedistinctfromthe2knownporcinecoronaviruses,trans-missiblegastroenteritisvir

3、usandhemagglutinatingencephalomyelitisvirus.In1946,DoYr.EandHUTC~I~GS(2)describedaviraldiarrhea,inswineandcalledittransmissiblegastroenteritis.Untilrecently,transmissiblegastroenteritisviruswastheonlyvirusknowntobespecificallyassociatedwithdiarrheainswineofallages.In1976,followingthediscover

4、yofrotavirusesindifferent,animalspecies,aporcinerotaviruswasdetectedinthefecesofpigswithdiarrhea(14).Diarrheacouldbereproducedexperimentallyinpigletswiththisvirus.InasearchforrotavirusesonBelgianswinebreedingfarmswithdiarrhealproblems,anewcoronavirus-likeparticlewasdetectedbyelectronmicrosco

5、picexaminationofintestinalorfecalsamplesfromsickpigs.Thepresentreportdescribesthemorphologyofthiscoronavirus-likeparticle,andshowsthatitisdistinctfromtheknownporcinecoronavirusesandcausesdiarrhea.Uptonow,theonlycoronavirusesisolatedfromswinehavebeentransmissiblegastroenteritisvirus{TGEV)andh

6、emagglutinatingencephalomyelitisvirus(HEV).TGEVhasbeendescribedasacauseofdiarrheainswineincountriesallovertheworld(13).Numerousstudieshavebeenperformedonthevirus--animalinteractionsofTGEV,whichisusuallydetectedeitherbyitsisolationfromfecalThesestudiesweresupportedbytheInstitutefortheEncourag

7、ementofl~e-searchinIndustryandAgriculture(IWONL),Brussels,Belgium.0304-8608/78/0058]0243/$01.00244M.B.PE~SAE~andP.~)EBOUC~::materialincellculturesorbyimmunofluorescenceinthesmM1intestinalepitheliumofinfectedpigs(7,12).TGEVinfectionscanalsobediagnos