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1、Chapter14DevelopmentofaSARSCoronavirusVaccinefromRecombinantSpikeProteinPlusDeltaInulinAdjuvantCliftonMcPherson,RichardChubet,KathyHoltz,YoshikazuHonda-Okubo,DaleBarnard,ManonCox,andNikolaiPetrovskyAbstractGivenperiodicoutbreaksoffatalhumaninfectionscausedbycoronaviruses,developmento

2、fanoptimalcoronavirusvaccineplatformcapableofrapidproductionisanongoingpriority.Thischapterdescribestheuseofaninsectcellexpressionsystemforrapidproductionofarecombinantvaccineagainstsevereacuterespiratorysyndromecoronavirus(SARS).Detailedmethodsarepresentedforexpression,puriTcation,a

3、ndreleasetestingofSARSrecombinantspikeproteinantigen,followedbyadjuvantformulationandanimaltesting.ThemethodshereindescribedforrapiddevelopmentofahighlyprotectiveSARSvaccineareequallysuitedtorapiddevelopmentofvaccinesagainstotherfatalhumancoronavirusinfections,e.g.,theMERScoronavirus

4、.KeywordsCoronavirus,SARS,MERS,Vaccine,Adjuvant,Deltainulin,Advaxadjuvant,Baculovirus,Manufacture,cGMP1IntroductionThesevereacuterespiratorysyndromecoronavirus(SARS)wasTrstidentiTedin2003afteraseriesoffatalpneumoniacaseschar-acterizedbyanin?ammatorycellinTltratewithdiffusealveolardam

5、age[1]startedinHongKongbeforespreadingtoothercoun-tries[2].Beforebeingcontrolledbyquarantinemeasures,~8000humanswereinfected,withfatalityashighas50%intheelderlyandanoverallcasefatalityrateof~10%[2].Morerecently,theMiddleEastrespiratorysyndromecoronavirus(MERS)hascausedaseriesofseriou

6、sand,insomecases,fatalhumaninfections[3].Giventheriskoffutureserioushumancoronavirusoutbreaks,developmentofasuitablevaccineplatformtoprotectagainstsuchvirusesisamajorpriority.Thesevaccinespresentseveralchallengesincludingtherapiditywithwhichtheseoutbreaksdevelopandhencetheneedforrapi

7、dvaccinemanufacture.Hence,asuccessfulcoronavirusvaccineplatformmustovercomemultiplechallenges.SunilThomas(ed.),VaccineDesign:MethodsandProtocols:Volume1:VaccinesforHumanDiseases,MethodsinMolecularBiology,vol.1403,DOI10.1007/978-1-4939-3387-7_14,?SpringerScience+BusinessMediaNewYork20

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