《冠狀病毒Coronavirusl論文-2013 Antiviral effects of a probioticEnterococcus faeciumstrain against transmissible gastroenteritis coronavirus.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。

1、ArchVirol(2013)158:799807DOI10.1007/s00705-012-1543-0ORIGINALARTICLEAntiviraleffectsofaprobioticEnterococcusfaeciumstrainagainsttransmissiblegastroenteritiscoronavirusWeidongChai?MichaelBurwinkel?ZhenyaWang?ChristianePalissa?BettinaEsch?SvenTwardziok?JulianeRieger?PaulWrede?MichaelF.G.Schmi

2、dtReceived:24July2012/Accepted:7October2012/Publishedonline:28November2012óSpringer-VerlagWien2012AbstractTheenteropathogeniccoronavirustransmissi-Introductionblegastroenteritisvirus(TGEV)causesseverediseaseinyoungpiglets.WehavestudiedtheprotectiveeffectsoftheTransmissiblegastroenteritisvir

3、us(TGEV)infectsentericprobioticEnterococcusfaeciumNCIMB10415(E.fae-andrespiratorytissuesandcausesseveregastroenteritiscium),whichisapprovedasafeedadditiveintheEuropeanwithamortalityratecloseto100%innewbornpiglets[3,Union,againstTGEVinfection.E.faeciumwasadded36].Theappearanceofthecloselyrel

4、atedTGEVvarianttoswinetesticle(ST)cellsbefore,concomitantlywith,porcinerespiratorycoronavirus(PRCoV)hasbeenfoundorafterTGEVinfection.Viabilityassaysrevealedthatbene?cialinpreventingTGEVinfections,possiblythroughE.faeciumledtoadose-dependentrescueofviabilityofinductionofneutralizingantibodie

5、sthatcanprovidecross-TGEV-infectedcellsreachingnearlytocompleteprotec-protectionagainstTGEVinfection[34,39].However,TGEtion.VirusyieldsoftheE.faeciumtreatedcultureswereprevalenceisstillbeingreported,andsomeTGEVstrainsreducedbyuptothreelog10units.Westernblotanalysisofhavebeenisolatedfromdome

6、sticpigsindifferentpartsofpuri?edTGEVrevealedthatthelevelsofallviralstructuraltheworld[28].Commerciallyavailablevaccines,eitherproteinswerereducedafterE.faeciumtreatment.Usinginactivatedorattenuated,havefailedtoprovidefullpro-transmissionelectronmicroscopy,weobservedattachmenttectiontopigle

7、ts[38].ItislikelythattheparentallyappliedofTGEVparticlestothesurfaceofE.faeciumwhichmightinactivatedvirusesdonotinducethelocalimmunebeameanstotrapvirusandtopreventinfection.Increasedresponseinthesmallintestinethatisrequiredforprotec-productionofnitricoxi