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1、VIRALIMMUNOLOGYOriginalArticlesVolume26,Number2,2013aMaryAnnLiebert,Inc.Pp.126–132DOI:10.1089/vim.2012.0076ImmunogenicityandProtectionEf?cacyofMonomericandTrimericRecombinantSARSCoronavirusSpikeProteinSubunitVaccineCandidates1,22221JieLi,LauraUlitzky,EricaSilbe

2、rstein,DeborahR.Taylor,andRaphaelViscidiAbstractSevereacuterespiratorysyndrome(SARS)isanewlyemerginginfectiousdisease,andaneffectivevaccineisnotavailable.Inthisstudy,wecomparedtheimmunogenicityandprotectionef?cacyofrecombinantproteinscorrespondingtodifferentdom

3、ainsoftheSARS-coronavirusspikeprotein.TrimericrecombinantproteinswerecreatedbyfusingthefoldondomainderivedfromT4bacteriophagetothecarboxy-terminiofindividualdo-mainsofthespikeprotein.Whilethefull-lengthectodomain(S)ofthespikeprotein,thefull-lengthectodomainfuse

4、dtofoldon(S-foldon),theS1domain(S1),S1-foldon,andtheS2domain(S2)antigensallelicitedcomparableantibodytitersasmeasuredbyELISA,S-foldoninducedasigni?cantlyhighertiterofneutralizingantibodyandS2proteindidnotelicitvirusneutralizingantibodies.Whentestedinamousevirus

5、replicationmodel,allthemicevaccinatedwiththeS1,S1-foldon,S,orS-foldonwerecompletelyprotected.IntroductionsideredamaintargetforSARSvaccines(6).Inthepresentstudy,wecomparedthefull-lengthectodomainofSproteinSevereacuterespiratorysyndrome(SARS)-associatedanditsfrag

6、ments(S1andS2domains)withrespecttoim-coronavirus(SARS-CoV)isanemerginginfectiouspath-munogenicityandprotectionagainstviralinfectioninmice.ogenofzoonoticorigin.AlthoughnonewcasesofSARShaveInitsnativestate,Sproteinisatrimer;however,whenitsbeenreportedsince2004,SA

7、RShasthepotentialtore-emergeectodomainisexpressedasarecombinantproteinineu-fromananimalreservoirorthroughaccidentalorintentionalkaryoticsystems,theproteinexistspredominantlyinamo-release.Therefore,thedevelopmentofeffectivevaccinesisnomericform(7).Tomaketrimeric

8、recombinantspikehighlydesirableforthepreventionandcontainmentoffutureproteins,weexploiteda27-aminoacidsequence,calledtheoutbreaksofSARS.foldondomain,whichwasidenti?edintheba