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1、LETTERdoi:10.1038/nature12711IsolationandcharacterizationofabatSARS-likecoronavirusthatusestheACE2receptor11122231Xing-YiGe*,Jia-LuLi*,Xing-LouYang*,AlekseiA.Chmura,GuangjianZhu,JonathanH.Epstein,JonnaK.Mazet,BenHu,111111145WeiZhang,ChengPeng,Yu-JiZhang,Chu-MingLuo,BingTan,NingWang,YanZhu,GaryCramer

2、i,Shu-YiZhang,4,621Lin-FaWang,PeterDaszak&Zheng-LiShiThe2002–3pandemiccausedbysevereacuterespiratorysyndromeinKunming,YunnanProvince,China(ExtendedDataTable1).Atotalcoronavirus(SARS-CoV)wasoneofthemostsignificantpublichealthof117analswabsorfaecalsampleswerecollectedfromindividualbats15,14eventsinrec

3、enthistory.AnongoingoutbreakofMiddleEastrespira-usingapreviouslypublishedmethod.Aone-stepreversetranscrip-2torysyndromecoronavirussuggeststhatthisgroupofvirusesremainstion(RT)-nestedPCRwasconductedtoamplifytheRNA-dependentakeythreatandthattheirdistributioniswiderthanpreviouslyrecog-RNApolymerase(RdR

4、P)motifsAandC,whichareconservedamong15nized.Althoughbatshavebeensuggestedtobethenaturalreservoirsalphacoronavirusesandbetacoronaviruses.3–5ofbothviruses,attemptstoisolatetheprogenitorvirusofSARS-Twenty-sevenofthe117samples(23%)wereclassedaspositivebyCoVfrombatshavebeenunsuccessful.DiverseSARS-likeco

5、rona-PCRandsubsequentlyconfirmedbysequencing.Thespeciesoriginofviruses(SL-CoVs)havenowbeenreportedfrombatsinChina,allpositivesampleswasconfirmedtobeR.sinicusbycytochromeb5–816EuropeandAfrica,butnoneisconsideredadirectprogenitorsequenceanalysis,asdescribedpreviously.AhigherprevalencewasofSARS-CoVbeca

6、useoftheirphylogeneticdisparityfromthisvirusobservedinsamplescollectedinOctober(30%in2011and48.7%inandtheinabilityoftheirspikeproteinstousetheSARS-CoVcellular2012)thanthoseinApril(7.1%in2011)orMay(7.4%in2012)(Extendedreceptormolecule,thehumanangiotensinconvertingenzymeIIDataTable1).AnalysisoftheSpro

7、teinRBDsequencesindicatedthe9,10(ACE2).Herewereportwhole-genomesequencesoftwonovelbatpresenceofsevendifferentstrainsofSL-CoVs(Fig.1aandExtendedcoronavirusesfromChinesehorseshoebats(family:Rhinolophida