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1、EuropeanJournalofPhycologyISSN:0967-0262(Print)1469-4433(Online)Journalhomepage:http://www.tandfonline.com/loi/tejp20IsolationandregenerationofprotoplastsfromPorphyradentataandPorphyracrispataErwanArGall(YvanLeGall),Young-MengChiang&BernardKloaregTocitethisarticle:

2、ErwanArGall(YvanLeGall),Young-MengChiang&BernardKloareg(1993)IsolationandregenerationofprotoplastsfromPorphyra?dentataandPorphyra?crispata,EuropeanJournalofPhycology,28:4,277-283,DOI:10.1080/09670269300650391Tolinktothisarticle:https://doi.org/10.1080/0967026930065

3、0391Publishedonline:24Feb2007.SubmityourarticletothisjournalArticleviews:379Citingarticles:14ViewcitingarticlesFullTerms&Conditionsofaccessandusecanbefoundathttp://www.tandfonline.com/action/journalInformation?journalCode=tejp20Eur.J.Phycol.(1993),28:277-283.Printe

4、dinGreatBritain277IsolationandregenerationofprotoplastsfromPorphyradentataandPorphyracrispataERWANARGALL(YVANLEGALL)1'2,YOUNG-MENGCHIANG1ANDBERNARDKLOAREG1InstituteofOceanography,NationalTaiwanUniversity,Taipei,Taiwan,RepublicofChina2Centred'EtudesOc~anographiquese

5、tdeBiologieMarine,CNRS(UPR4601)andUniversit~ParisVI,B.P.7429682RoscoffCedex,Brittany,FranceLargenumbersofprotoplasts(106to3.2x107pergramfleshweight)wereroutinelyisolatedfromtwoTaiwanesespeciesofPorphyra:P.dentataandP.crispata.Intermediateandfinalformsofregeneration

6、wereobserved,includingswollencells,monospore-likecells,filaments,callus-likestructuresandbladelets.RegenerationofleafyplantswasachievedfromprotoplastsofP.crispata,whereasonlyconchocelis-likefilamentswereregeneratedfromprotoplastsofP.dentata.Inthislatterspecies,conc

7、hosporangiaandmonosporangiaappearedonthefilamentsuponcultivationat25°C.Thevariabilityinregenerativepatternsisdiscussed.Keywords:Isolation,Porphyra,protoplasts,regeneration,Taiwan.IntroductioncommonspeciesalongthecoastsofTaiwan.Bothexhibitmicroscopicallydentateblade

8、s.ThefoliosethallusisbroadAsisolatedplantcellswithoutacellwall,protoplastsaretolinearlanceolateanddioeciousinP.dentata,whereasitisusefultoolsinst