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1、PLoSOne.2013;8(9):e74363.Publishedonline2013September3.doi:10.1371/journal.pone.0074363PMCID:PMC3760846PinelliapedatisectaAgglutininTargetsDrugResistantK562/ADRLeukemiaCellsthroughBindingwithSarcolemmalMembraneAssociatedProteinandEnhancingMacrophagePhagocytosisAgglutininTargetsDrugResis

2、tantK562/ADRLeukemiaCellsthroughBindingwithSarcolemmalMembraneAssociatedProteinandEnhancingMacrophagePhagocytosisAgglutininTargetsDrugResistantK562/ADRLeukemiaCellsthroughBindingwithSarcolemmalMembraneAssociatedProteinandEnhancingMacrophagePhagocytosisAgglutininTargetsDrugResistantK562/

3、ADRLeukemiaCellsthroughBindingwithSarcolemmalMembraneAssociatedProteinandEnhancingMacrophagePhagocytosisAgglutininTargetsDrugResistantK562/ADRLeukemiaCellsthroughBindingwithSarcolemmalMembraneAssociatedProteinandEnhancingMacrophagePhagocytosisCollegeofLifeSciences,ZhejiangSci-TechUniver

4、sity,Hangzhou,Zhejiang,ChinaAgglutininTargetsDrugResistantK562/ADRLeukemiaCellsthroughBindingwithSarcolemmalMembraneAssociatedProteinandEnhancingMacrophagePhagocytosisKanChen,XinyanYang,LiqinWu,MeilanYu,XiaoyanLi,NaLi,ShuanghuiWang,andGongchuLi*SophiaNKaragiannis,EditorAuthorinformation

5、?Articlenotes?CopyrightandLicenseinformation?Goto:AbstractPinelliapedatisectaagglutinin(PPA)haspreviouslybeenusedinlabelingfractionsofmyeloidleukemiacellsinourlaboratory.WereportherethatabacterialexpressedrecombinantPPAdomainbtaggedwithsolublecoxsackieandadenovirusreceptor(sCAR-PPAb)pre

6、ferentiallyrecognizeddrugresistantcancercellsK562/ADRandH460/5Fu,ascomparedtotheirparentalcelllines.PretreatmentofK562/ADRcellswithsCAR-PPAbsignificantlyenhancedphagocytosisofK562/ADRbymacrophagesinvivo.Meanwhile,inaK562/ADRxenograftmodel,intratumoralinjectionofsCAR-PPAbinducedmacrophag

7、einfiltrationandphagocytosis.Furthermore,immunoprecipitation,massspectrometryandWesternblotidentifiedthemembranetargetofPPAonK562/ADRassarcolemmalmembraneassociatedprotein(SLMAP).AnantibodyagainstSLMAPsignificantlypromotedthephagocytosisofK562/ADRbymacrophagesinvitro.Thesefindi